TitleThe effects of temperature, pH and concentration on enzymes.IntroductionA catalyst is a substance that regulates and accelerates the rate of a biochemical reaction but is not consumed or modified in such reactions. Enzymes are globular proteins that act as biological catalysts. Enzymes cannot make a reaction happen, they simply accelerate the reaction from 100,000 times faster to over a billion times faster (Brooker 2012). They speed up the reaction by lowering the activation energy – the amount of energy needed to push a reactant to the level needed for action. The activity of an enzyme is very chemically specific; some enzymes control only a specific reaction. In an enzyme-catalyzed reaction, the substance that an enzyme acts on is called a substrate, and the site where the substrates fit is known as the active site (Marieb and Hoehn 2013). The process by which enzymes bind to the substrate can be seen as a key. Only a key can open a lock. Peroxidase is an enzyme that helps promote the oxidation of various compounds using natural peroxides such as hydrogen peroxide (H2O2) which is reduced to water. Peroxidase breaks down peroxides created from the byproducts of biochemical reactions within the body into harmless products.HROH + H2O2 R=O + 2H2OGuiacol Hydrogen peroxide Oxidized guiacolIn this experiment, we examined what will happen to peroxidase when placed in different pHs, concentration and temperature. Peroxidase, an enzyme that catalyzes the reaction of hydrogen peroxide, will work best in the pH range of 5.0 to 9; will be slowed down at high temperatures and concentrations.Methods and MaterialsMaterialsTubes (50), Spectronic 20 (Spec. 20), Distilled water, Guaiacol, Hy......half of paper......temperature and pH of the amylase enzyme supports the hypothesis. From his result, it was found that amylase works faster at a certain pH range and works better at temperatures of 37°C (cite). A follow-up experiment could be done to see what would happen if the concentration of the substrate changed instead of that of the enzyme. ReferenceBrooker, Robert J. 2012. “Overview of Genetics in Chapter 1 Concepts of Genetics. McGraw-Hill, New York. “Effect of temperature and PH on amylase.” 2014Fregeau,MR., M.Hoover, E.Maney [ Eds].2012. Photosynthesis and Scientific Writing, Chapter 8 in Biology 132 Introduction to the Textbook Academx Publishing, Virginia Beach VA 138pp.Marieb, Elaine N., Hoehn , Katja 2013. “Chemistry Comes to Life in Chapter 2 Human Anatomy and Physiology . Pearson Education, Inc. Glenview, IL 50Pp