In the first experiment, our results supported the hypothesis that T. zuluensis reacts differently to light exposure. However, it didn't grow better in the dark. Pathogen growth was significantly different with different incubation volumes in the light and dark. Growth under 25% light volume was significantly higher than all other light intensities and darkness in each media treatment (Table 5). Our results also showed that there is a significant difference in mushroom growth after exposure to different light intensities. Incubation in different volumes of light revealed different diameter growth of T. zuluensis. Growth was lower in darkness and 75% light, but greater at 25% light volume. He indicated that the pathogen needs light but with limited intensity to grow quickly. Dark incubation showed the lowest growth across all light volumes. Say no to plagiarism. Get a tailor-made essay on "Why Violent Video Games Shouldn't Be Banned"? Get an original essay However, light preferences vary between different mushrooms as some mushrooms grow best in darkness and others prefer light conditions. Hatakeyama et al. , 2017 found that light exerted negative effects on the diameter growth of A. oryzae and that conidia production was also repressed in light incubation. In many fungi, the light pulse is also necessary to initiate the formation of conidia, but many other mechanisms are also involved in its regulation. Similarly, different growth stages also regulate the initiation of conidia and the processes that stimulate enzyme production (Tisch and Schmoll, 2010). Biomass production is also another imperative parameter that can be compared in different aspects in future research since it is also regulated by light and darkness (Murthy et al., 2015). In their research, the biomass of A. oryzae increased by 11% in the dark compared to the light. Light often induces or suppresses spore production in fungi and can have various effects on reproductive development, growth and metabolism (Atoui et al., 2010). In addition to the observed effects on growth in light and darkness, it also affects the nutritional balance of fungi (Carlile, 1965). The availability/use of substrates by fungi is closely linked to exposure to sunlight (Friedl et al., 2008). The regulation of light not only affects the utilization but also the absorption of substrates which ultimately affects the entire metabolic process of the cell since the corresponding compounds are missing (Hill, 1976). Limitation of these essential compounds inhibits fungal growth. (Tisch and Schmoll, 2010) provided the example that glucose uptake in A. ornatus was significantly inhibited when incubated in light (Hill, 1976), yet induced the formation of conidia. The interrelationship between glucose and conidia formation was highlighted by the finding (Betina and Zajacova, 1978) that increased glucose concentration reduces conidia production but does not affect growth. In other studies (Graafmans, 1976), light stimulated the synthesis of polysaccharides in Penicillium isariiforme. The fungal cell wall is the first site exposed to photons after illumination (Tisch and Schmoll, 2010). Several studies revealed where light altered cell wall composition and doubled the amount of chitin in A. giganteus compared to dark incubation (Fiema, 1983). A temporary alteration was also observed in the cell wall structure of P. blakesleeanus in research conducted by Estrella and Herrera,.