A modern study releases a method in which the amino acid is detected via a kit. This kit is used to detect a special amino acid which is hydroxyproline. It is detected for the purpose of improving the quality of the foods for which it is used such as in the food industry. Different color reactions are also used to detect it as ninhydrin. But it is more specifically used to detect peptides and proteins. The amino acid detected as hydroxyproline is used as a marker to detect specific diseases. 3-Hydroxyproline is used to detect cancers such as lung or breast cancer, etc. It is the form of hydroxyproline. 4-Hydroxyproline is another form that is used to detect bone disease. It is used as a marker to detect tumors and many other diseases, etc. The method of identifying 3-hydroxyproline by a graphic form of ninhydrin is incorrect, but some other amino acids can be detected with this method. It can be detected through a process known as HPLC. But it is not a pure synthesis method due to the numerous columns. It is also a time-consuming method. But nowadays it is necessary to separate hydroxyproline quickly and easily. The method used to detect it easily is “kit detection”. This kit specifically detects hydroxyproline-3 and 4. And is identified through a process of absorbance and light spectrum in the form of curves. This process used solid phase chemicals. Also detects nitrobenzene reagents. It also detects amino acids through the use of a buffering agent. Say no to plagiarism. Get a tailor-made essay on "Why Violent Video Games Shouldn't Be Banned"? Get an original essay Further studies produce another method to find the amino acid by first detecting the amino acid from nitrobenzene from the solid phase. Then let it react with hydroxyproline and finally find the shape of the absorbance curve due to this reaction. This reaction is temporal and indicates whether hydroxyproline is present or not. If it is present, nitrobenzene reacts with it. The solid phase contains a buffering agent and an acidic solution. The reaction that occurs between them provides us with information on its structure. Another 20 amino acids can also be detected with this method. Amino acid detection can also be detected using different reagents and detection kits. Hydroproline is detected by a hydroxyl group. The 3,4,5-hydroxyproline found by this method. It is also used as an indicator for disease detection. This method found its L form, but further studies also reveal its D form. This is found in solution and contains water, glycerin etc. If the concentration of the solution is higher than that of the solvent, it moves out of the solution. While if it is low then release it in the same way. A specific wavelength is adjusted when running the fluorescence spectrum. The same procedure applies to the absorption spectrum. When the two forms of hydroxyproline are present in a solution, it is possible to detect both because it is not possible to distinguish between the two spectra used. It is found in the urine sample. It is detected using different reagents such as; HPLC, L-proline. Other amino acids can also be detected by different processes such as; spectrum and chromatography etc. The amino acid cannot move in a solid phase. Different types of apparatus like test tubes, watch glasses, glasses and other things etc are used in it. Different types of vehicles are also used. Under various conditions it is collected as a sensing agent; NBD-Cl. Hydroxyproline reacts with NBD-Cl and perfects its reaction that occurs above. Formaintain the solvent in which hydroxyproline is located, the pH is maintained at 4.0 – above 8.5. It is maintained by a buffering agent process which can be solid or liquid. The reaction time is approximately 2 minutes. While it is also incubated at room temperature or between 20 and 50 C. Some inorganic or organic chemicals are used to detect acids. This reaction occurrence ratio is 1:1. It does not depend on neutralization or any other type of reaction. The reaction product has a wavelength of 500. Its absorbance value is approximately 500 nm and no limit is applied. The absorbance spectrum has a wavelength of approximately 350-660 nm. While others have 250-550 nm. The curve represents the presence of hydroxyproline and is found through urine. Examples include the formation of a sample solution in which several amino acids are found. Drawing of the reaction in the form of a spectrum. Using all these methods hydroxyproline is detected without any manipulation. It is used in industries to examine the sample at a specific time. It is detected through the reagents in Claim 1. While in Claim 2 it is detected through the spectrum. It is detected via the reaction in claim 3. In claim 4 it is detected via kit. Its L shape is used in the formation of medicines. It is prepared in a cultural medium. It is the synthesis of proteins. This method is not used in industrial preparation because it is found in four isometric forms. It is prepared and obtained from animal tissues. Several steps are used to extract it. It is prepared in the industry using the cultural media method. It was found to meet the requirements of organisms. It is present in our body, so if we take it as it is it causes an overdose, so its best form is Vitamin C. It is taken as Vitamin C. It is given to us because of its requirement in our body. muscles, ligaments, tendons, etc. It is also essential for fish muscles, etc. Collagen is a hydroxyproline and is not soluble in an alkaline environment. It also exists in nature and is found in feces. It is also a source of protein. It is soluble in acids. Its deficiency causes diseases such as; compartment syndrome, polymyositis, myopathy etc. It also has effects on ligaments, cartilage, cardiovascular diseases, venous insufficiency and respiratory diseases etc. It is also present in functional foods, dairy products and health drinks etc. Requires vitamin C as a cofactor. Collagen plays a role in the stability of the triple helix. Trans-4-hydroxy-L-proline is the form of 4-hydroxyproline. One of its forms is used as in cosmetic compounds. These compounds provide benefits to the skin. It is good for dry skin. Protects our skin from radiation, etc. N-acetyl-hydroxyl-l-proline is prepared from gelatin. It is used to treat connective tissue disorders. It is obtained from hydroxyl-L-proline. Its method of separation from the other four forms is expensive. It is the normal component of many proteins but gelation is used for many industrial preparations. It can also be prepared chemically but it doesn't matter. It is also prepared by a method in which arginine is removed, but this method is very expensive. The main result of this research is that arginine is not removed and it is less expensive and good yield is obtained after such process. You get a greater amount of return than the above. Collagen contains polypeptides. It is composed of 300 tripeptide sequence repeats. The single collagen molecule is in the form of a triple helix. The stability between them is not due to hydrogen bonding because it is not present in it. It's due to the Water Bridge. AND,.